1,4-Dioxane (1,4-D) has been classified as a human carcinogen. Microbial degradation of 1,4-D would be an attractive bioremediation approach. In this study, the kinetics of 1,4-D biodegradation was determined in a batch reactor by a mixed consortium of bacteria isolated from industrial sludge contaminated with 1,4-D. As a result, the kinetic coefficients of Monad equation were found to be qm = 0.0051 mg 1,4-D/mg VSS.hr, Ks = 7.47 mg/L, kd = 0.0023 mg/mg.hr, and Y = 0.41 mg VSS/mg 1,4-D. In addition, the 1,4-D degradation rate and specific degradation rate were increased with the increase in 1,4-D concentrations, ranging from 5 to 2000 mg/L, showing that the enriched culture has high affinity to 1,4-D biodegradation.