Foot and mouth disease (FMD) is a highly infectious disease that affects cattle, ruminant animals causing serious economic losses and animal breedings worldwise. In this study, a DNA vaccine was developed candidate that is based on the backbone of the expression vector pTandem1 (Novagen). This expression has a expression cassette containing a encoding region of VP1 epitop (141-160) of FMD virus cloning inframe with the gene encoding for Fc fragment of the heavy chain of pig IgG. To evaluate the DNA vaccine candidate, the plasmid DNA was transfected to CHO-K1 cell, protein expression was checked at transcription and translation level. Immune response of the plasmid was performed in Swiss mice. The results showed that VP1 antigen can express at the expression level of 0.4-0.7 pg/cell/day and the expression system in this study can induce immune response with a dose - dependent manner. This result will be the premise for the second phase of clinical trial in swine.