There are many methods for treating infertility patients, IVF plays an important role in the field of assisted reproduction and development are increasingly widespread in the world. However, the success rate in assisted reproduction only reached 30-40 percent. Currently, most are choosing embryos based on morphological criteria of the embryo. However, the morphological assessment not fully reflect the true quality of the embryos, if only based on morphological parameters, the results of in vitro fertilization treatment is limited. To improve the quality and refinement of some genetic diseases, Preimplantation Genetic Diagnosis (PGD) is one of the critical requirements, urgent and practical. On that basis, the authors conducted a study with project is "Study on embryo morphological changes after embryo biopsy for genetic diagnosis prior embryo transfer" to evaluate embryo morphological changes and assess some factors affecting the ability of embryo survival and create blastocyst after biopsy. Subjects and Methods: 102 surplus embryos, biopsy on day 3. Quality be evaluated based on the author Andres Salumets, 2001: on three main criteria: number of blastomeres in an embryo, cytoplasm fragments and the embryo volume. Results: After 24 hours biopsy survival rate of embryos, further development of the 3 groups of 93 embryos in total 102 embryos, reached 91.17 percent and after 48 hours blastocyst formation is 83, reaching 81.37 percent. Number of embryos that survived continued development (M and AB) account for 65.68 percent of 67 embryos. Debris cytoplasm fragment ratio, ZP thickness, embryo diameter between the groups did not change significantly. Conclusion: laser biopsy does not alter embryo morphology, viability and bag forming blastocyst. The more higher Maternal age, duration of infertility, FSH concentrations the less survival rate of embryos and less forming blastocyst. IVF or ICSI did not affect the survival rate of embryos, further development of blastocyst after embryo biopsy.