There were 710 E. coli, 272 K. pneumoniae isolates obtained in the Vietnam-Sweden Uong Bi hospital, Quang Ninh province. Among them, there were 30,3 percent E. coli, 54 percent K. pneumoniae strains producing extended spectrum beta-lactamase (ESBL) were detected by Double - disk method. Structural genes for TEM (34,1 percent), CTX-M (30,5 percent), SHV (24,7 percent) and OXA (10,7 percent) enzymes were detected by PCR method. The results of sequencing showed that: with TEM: TEM-1 (100 percent)
with CTX-M: CTX-M-15 (96 percent), CTX-M-79 (4 percent)
with SHV: SHV-1 (47,2 percent), SHV-11 (43,1 percent), SHV-83 (8,3 percent), SHV-28 (1,4 percent)
with OXA: OXA-1 (100 percent).