A study on biological indices was conducted on pupils of 16-18 years old at Que Vo No.1 high school in Bac Ninh province. The. study aimed at determining some physical and functional indexes of male and female pupils of 16-18 years old, contributing to establish the biological indexes of the present Vietnamese people. The results showed that there were differences in anthropometric measurements between male and female pupils as height, weight, bust, flexed right arm circumference. According to BMI index those pupils were classified as mean and lean physically, but for Pignet index all pupils were classified as weak physical/y. There were also differences in heart rate, blood pressure, and respiratory parameters between male and female pupils.