Five isolates capable of chitinase synthesis were isolated from soil's samples. By TLC analysis, it had shown that all five isolates hydrolyzed chitin to N-acetyl-chitobiose. Among these isolates, isolate MD 2-1 possesed the highest chitinase activity according to the results of enzyme diffusion on chitin containing agar plate and by chitinase assay. MD 2-1 was identified as Bacillus cereus with 99.5 percent identity by morphological characteristics and by API kit. The growth condition such as medium composition, initial pH, cultivation temperature and chitin concentration had pronounced effect on chitinase production of B. cereus MD 2-1. The results of the study revealed that the highest chitinase activity of 0.2 U/mL could be reached on MS- medium at initial pH medium 6.5, cultivation temperature 30oC and chitin concentration 0.3 percent after 24 hours cultivation.