Stem cells are defined by their capacity of self-renewal and generate functional differentiated cells that replenish lost cells throughout an organism's lifetime. In recent years human amniotic membrane has been recognised as a possible reserve source of stem cells that may be useful for clincal application in regenerative medicine. The aim of this study was to establish a new cell source that can be used for research and treatment
therefore, this study made isolation, proliferation cultures, and identification of expression for the markers of the stem cells isolated from human amniotic membrane. The results showed that the isolated cells in culture medium had a typical shape, and they expressed positive markers for stem cells and amniotic epithelial cells as Oct-4
SSEA4, Sox2, Vimentin, CK18. Those findings indicated that stem cells from human amniotic membrane were isolated and identified.