24 excellent bred lines (elite hybrid lines) in F8 and two mutant parents (Tam Xuan Dai mutant rice line No.3 - TXDDB3 and Du Hai Hau mutant rice lines No.5 - DHHDB5) cultivated to F9 in the tenth-month crop 2011. Leaves at the time of 30 days after implantation were used to extract DNA, using 31 SSR primer pairs belonging to different loci for analysis. PCR, electrophoresis PCR products. Also identified 23 agrobiological and grain quality characteristics. Data of electrophoresis PCR products and data on the agrobiological and quality characteristics of rice were said on the same processor, both were arialysed by Excel software version 5.0 and NTSYSpc2.1 to establish subgroups and set tree diagram reflected the genetic relationship among rice lines studied. Two tree diagram fonns has similar groups, in the.case of genetic diversity at the molecular level (DNA) is 7, but in other case the remaining 8 groups. The line composition of the entire group is basically the same. The results showed that the genotypic diversity in the molecular level (DNA) reflects the diversity of phenotypes corresponding to the agro-biological characteristics and quality of 24 rice hybrid lines and two mutant lines used as parents.