Nghiên cứu tách dòng và biểu hiện protein tái tổ hợp cfp10/esat6 của mycobacterium tuberculosis

• Tác giả: Đức Anh Đặng, Thị Quỳnh Nga Đỗ, Thị Phượng Hoàng, Le Thanh, Thị Hải Âu Trần, Thị Kim Liên Vũ
• Ngôn ngữ: vie
• Ký hiệu phân loại: 616.99 Tumors and miscellaneous communicable diseases
• Thông tin xuất bản: Y học dự phòng, 2012
• Mô tả vật lý: 91-95
• Bộ sưu tập: Metadata
• ID: 637154

Tóm tắt

A DNA encoding the 10 Kdal culture protein (CFP10) and 6 Kdal Early Secreted Antigenic Target (ESAT-6) of M. Tuberculosis was cloned and inserted into a bacterial expression vector of pET21a resulting a 6 hexahistidine tag-CFP10-ESAT6 fusion gene construction. This plasmid was transformed into E. coli strain BL21 to an expression recombinant protein. Fusion protein CFP10-ESAT6 was purified by Ni-NTA (HisPur Ni-NTA Purification Kit). The concentration of recombinant protein was determined by Bradford method. SDS-PAGE and Western Blot analysis demontrated that self - made recombinant protein CFP10-ESAT6 was highly specific for anti_ESAT6 antibody as well as antiserum of patient with tuberculosis, but showed no reaction with antiserum of healthy people.