Following the research on producion of antibody against recombinant Beta-subunit of FOF1-ATPase, in this study, the authors reported the generation of complex of chromatophore extracted from Rhodospirillum rubrum with quantum dots
these are essential requirements for biosensor development. R. rubrum cells were disrupted by French pressure combines ultrasonication to release the chromatophore. Chromatophore was purified by density-gradient ultracentrifugation. The pigmented bands were collected and examined by electron microscope with magnification of 15,000X - 30,000X. The results showed that chromatophore shaped like discs and cups 100 nm in diameter. The highly purified chromatophore was then mixed with red QDs, 2-3 nm in size, in suitable conditions to form complex of chromatophore-QDs. The formation of complexes was confirmed by luminescence intensity under fluorescent microscope. The complex will be used for further research to develop the biosensor.