Four compounds including 6,7-dimethoxy coumarin (1), 3,3',4'-tri-O-methoxy ellagic acid (2), methyl gallat (3) anduracil (4) were isolated and identified for the first time from the bark of Euphorbia tithymaloides (P.). The ethanol extractshowed significance of cytotoxic effect against lung cancer cell (LV-I) with IC50 value of 3.01 ug/ml.