The present study was conducted to survey on collection of feeder cell types for in vitro culture of embryos. In experiment I, oviduct cell clusters were collected from pubertal sows. The amount of clusters with good morphology and activity obtained from good-looking big oviducts with few blood vessels was higher than that from good-looking, small ones. and ones with many vessels. After freezing and thawing, oviduct cells kept their good quality similar to non-freezed ones. In experiment 2, fibroblasts were collected from mouse fetuses and cultured in DMEM 10 percent FBS (37°C, 5 percent CO2, 100 percent humidity). The mean number of fibroblasts obtained was 17.76 million cells per fetus. Growth speed of freezed-thawed fibroblasts were not different in compare to non-freezed cells. In conclusion, collection and evaluation of cells before and after freezing were conducted in 2 types of cells. The present study would contribute modestly to establishment of in vitro culture system improvement towards biomedical researches in the future.