Bacterial wilt is one of the most serious diseases of tomato in tropical and subtropical areas. It is now the major constraint on production of tomato in many parts of Vietnam. Bacterial wilt is caused by the soil borne bacterium and Ralstonia solanacearum. This oomycete pathogen attacks on leaves, stems, and roots of tomato. Introduction of resistant varieties is therefore the most effective measure to control this disease. However, conventional breeding approach for disease resistance is difficult, labor cost and time consuming. The objective of this study is to identify genetic polymorphism of BW bacteria using molecular marker SSR for identifying resistant tomato varieties to Ralstonia solanacearom isolate collected in Vietnam. The result of phenotypic evaluation by artificial infection showed that Vimina 1 and Vimina 2 varieties exhibited resistance to 3 isolates RsDT6, RsDT3 and RsDD1. 3 isolates are exhibited the differential virulence. Varieties of Terminator, Tropic, Tomato and TN 323 are moderate resistance to RsDT3 isolate. Seven varieties which include Safina 404, Red Crown 250, Terminator, Mongal (T11), Tropic, Caribo and TN 323 are moderate resistance to RsDD1 isolate. The result also identified the marker SSR 306 on chromosome 4, ranged from 51 cM to 71 cM showing polymorphic between resistant varieties (Vimina 2) and susceptible variety (Seeda tomato) with a band of 290bp and 258bp. This result opens up for marker assisted selection of bacterial wilt resistant improvement of tomato varieties in Vietnam.