Research was carried out to produce virus-free plants via meristem culture of Allium wakegi. The explants were apical meristems (0.3 - 0.5mm) derived from virus infected plants. The results indicated that MS medium supplemented with 0.75 mg/l BA and 0.5 mg/l IAA was the most suitable medium for in vitro shoot regeneration from meristem, the rate of regenerated shoots was 73.33 percent. The highest rate of shoot propagation was 2.0 times with type I shoot and 4.07 times with type II shoot after 4 weeks, on MS medium containing 1.5 mg/l BA. The shoots were transferred to rooting media by addition qf 0.5 mg/l IAA and the rate of root formation was 100 percent, the average number of root was 4.33. RT-PCR method with primers Clfor/CIRev showed that the regeneration plants from meristem were virus-free.