Anaerobic digestion of piggery manure generates effluents which containing high concentration of inorganic nitrogen and phosphorus and causes eutrophication when discharged into natural water bodies. Microalgae can be cultured in an anaerobic effluent as a required tertiary treatment as well as for biodiesel feedstock production. In this study, anaerobic digested piggery effluent was used as nutrients source for culturing green rnicroalgae Chlorella vulgaris. The effect of dilution of the anaerobically digested piggery effluent nutrients removal efficiency of Chlorella vulgaris was determined. During 8 days in batch culture, Chlorella vulgaris grew faster in the more diluted wastewater, which related to initial COD concentration of about 400 mg/L. The maximum specific growth rate and biomass concentration were 0.306/day, and 0.191 g/L, respectively. Microalgae removed ammonia, total phosphorus and COD by 60-95.8 percent, 22-68 percent and 34-73.8 percent, respectively. The lipid contents of algal biomass after 8 day were 28-30 percent of dryweight, which could be utilized as a feedstock for biodiesel production.