All natural products of Piper betle L. trunks were extracted by using solvent methanol. Next, this extraction was distributed in n-hexane then diclomethane and ethylacetate. Their extraction removed solvent by vacuum obtained crude n-hexane (H, 3,75 percent), diclomethane (D, 2.91 percent) and ethylacetate (E, 1.6 percent). The diclomethane and ethyl acetate extracts showed significant inhibition against S. Aureus and E. coli and Asp. niger. From these extracts, stigmast-4-en,3,6-dione (1), eugenol (2), 4-allylpyrocatechol (3), 4-allylphenol (4) and aristololactam A-II (5) have been isolated. The structure of these compounds have been elucidated on the basis of spectral studies: IR (infrared), MS (mass spectrometry), nuclear magnetic resonance proton and carbon (1H-NMR), and the spetrometric 2D such as HSQC and HMBC. Isolation of compounds 1,4 and 5 from this source is being reported here for the first time.