Maize (Zea mays L.) is an important crop after rice which has been cultivated on soils of Eastern of South Vietnam, however maize plant needs to be used a lot of chemical fertilizers to ensure high yield therefore maize has often been planted on high fertility of soil. Besides that, nutrients from inorganic fertilizers, there are nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria in soils which can response nutrient requirement for maize growth and the aims of this study were (i) isolation and (ii) identification of these beneficial bacteria in soil rhizosphere of maize. Twenty-eight bacterial isolates having ability of nitrogen-fixing, phosphate and potassium solubilization were isolated on Burk without N, NBRIP and kaolinite media among ten isolates had high effectiveness and they were sequenced with 16S rRNA gene universal primers (8F and 1492R) and compared with Genbank database of NCBI by BLAST N software and MEGA 5.05 software was applied to analyse the phylogenetic tree. The results showed that 9 isolates are gram-negative bacteria (except isolate DN18 is gram-positive bacteria) which were identified as nitrogen-fixing bacteria and they are legume rhizobia, endophytes and PGPR (plant growth promoting rhizobacteria).