The rubber is the major industrial plant and having hight economic value, but the planting rubber in our country has been faced a nwnber of fungal diseases reducing the yield and quality of sap. One of the common diseases is branch dry disease caused by BotIyodiplodia theobromae Pat fungi. According to the investigation of the Rubber Research Institute this disease was most damaging in the rubber growing areas and can damage up to 100 percent. In this paper, the authors present some initial results on isolation, selection of microorganisms that has highly antagonistic action to fungi BotIyodiplodia theobromae Pat to control the branch dry disease of rubber. The result has been selected 06 strains BotIyodiplodia theobromae Pat that is TC1, QN1, MS1, SM1, YC1, ML2
these strains are highly toxic and were killed 100 percent rubber trees in the greenhouse condition
two strain C6- Bacillus subtilis and 51 - Bacillus amyloliquefaciens are biosafety and antagonistic against all 06 BotIyodiplodia theobromae Pat strains selected, diameter of corresponding inhibition ring was 13 - 15 mm and 14 - 16 mm. Two strains C6 and S1 have the potential to reduce the mortality rate of rubber trees from 100 percent to 20.8 percent and 25.0 percent in the greenhouse condition.