From 31 soil samples and wastewater in Khanh Hoa, 30 anaerobic strains were isolated. Among them, strain H23 with highest activities of protease, amylase and cellulose were selected. By 165 rRNA analysis, the H23 strains were classified at species level as Bacillus subtilis, respectively. And the 165 rRNA sequences of H23 is similarity with Bacillus subtilis ABll0598 of 99.9 percent, respectively. The optimum conditions for growth of strain Bacillus subtilis H23 are the carbon source: molasses, nitrogen source: soybean, pH of 7 and temperature 30°C and the maximum of OD620 nm of 2.32.