Escherichia coli 0157:H7 is a significant cause of foodborne illnesses and deaths in worldwide. A rapid and sensitive detection technique, which required to detect E. coli 0157:H7 in foods, is the important and indispensable. Shiga toxin 2 (encoded by slx2) is important virulence factor for 3TEC strains and E. coli 0157:H7 bacteria, therefore stx2 gene is the target gene for many detection tecnniques. In this study, the authors developed the loop mediated isothernal amplification (LAMP) method for rapid and sensitive detection of E. coli 0157:H7 strains. Primers were specially designed for founding on stx2 gene. The LAMP rection carries out in the therrnocycler or waterbath. The authors recognize no difference when used only inner primers (BIP/FIP) or 4-6 primers. A positive reaction was visualied when used SYBR green stain or under UV light.