Microfibrillated cellulose (MFC) was isolated from bamboo by two different methods. The first one is a combination of a thermomechanical treatment of bamboo culm called steam explosion and a mechanical extraction by grinding. In the second method, bamboo slabs were chemically treated with high concentration sodium hydroxide solution at elevated temperature following by grinding by stone miller. Obtained MFC were characterized in term of chemical composition, morphology and crystallinity. Two kinds of epoxy composites (EC) reinforced by woven glass fiber with addition of MFC were prepared. By comparison of the mechanical properties of these composites with those of the EC without presence of MFC, it is showed that addition of MFC resulted in little increase in tensile strength but remarkable improvement in tension-tension fatigue strength.