Spermatogonial stem cells (SSCs) are pluripotent and have properties similar to embryonic stem cells. In this experiment, the authors collected SSCs from young mouse testis and injected it into mature female mice which were irradiated with gamma rays to bring them to a near death state. The mice were irradiated with a dose of 900R to delete marrow, hematopoietic stem cells (HSCs). SSCs were injected into irradiated mice to determine potential differentiation of SSCs. The result showed that SSCs could prolong the life of the mice and increase the weight of mice that survived the radiation. Upon examination, we found cell division and an increase in the number of bone marrow cells. To prove the existence of transplanted SSCs in the irradiated mice, and isolated the DNA of some tissues and checked for the presence of Y chromosomes in females. Those organs which most often had SSCs were the heart, kidney and bone marrow
the liver and brain had the least amount of SSCs. This demonstrates that introduced SSCs divided and differentiated into some types of cells which replaced depleted cells. However, further study should be done to determine the potential differentiation of. SSCs in irradiated animals.