Tomato Yellow Leaf Curl Virus (TYLCV) is one of the main causes to decrease productivity and quality of solaniacease. The most effective method to prevent TYLCV is the use of resistant varieties. Recently, RNAi technology is a new trend in creating virus-resistant plants at many laboratories. The study obtained some significant achievements by generating these kinds of plant. In this report, the RNAi/pK7WGTLCV-CPi construct containing Vietnam TYLCV CP genes under controlling of CaMV35S was transformed into tabaccum plants (cv. K326) via Agrobacterium tumefaciens - mediated transformation. 101 pieces of leave were infected with A. tumefaciens carrying this transgene structure. After regeneration and selection we obtained 187 lines of transgenic, plants resistant to 50 mg/l kanamycin. Analyse the presence of trans genes in kanamycine-resistant plants by PCR with specific primers, The authors obtained 18/21 positive lines. These transgenic tabaccum are positive with RT-PCR analyse to test gene expression.