Tạo dòng ruồi giấm chuyển gen mang phức hợp uas-duch để nghiên cứu vai trò của protein uch-l1 đối với bệnh parkinson

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Tác giả: Thị Phương Thảo Đặng, Yamaguchi Masamistu, Nguyễn Thụy An Phan, Linh Thước Trần

Ngôn ngữ: vie

Ký hiệu phân loại: 616.8 Diseases of nervous system and mental disorders

Thông tin xuất bản: Công nghệ sinh học, 2012

Mô tả vật lý: 407-413

Bộ sưu tập: Metadata

ID: 640107

 Parkinson's disease (PD) is a progressive neurodegenerative movement 'disorder that affects the population older than 65 years of age and the death of dopaminergic neurons in PO patients lead to the impairment of movement. Nowaday, several studies have found mutant forms of genes linked to familial forms of PO which suggests that oxidative stress, mitochondrial dysfunction, impairment of the ubiquitinproteasome system, and protein aggregation may contribute to PO pathogenesis. Among them, UCH-L1 is one of the most abundant proteins in the brain and plays an important role in ubiquitin-proteasome system in order to degrade the unfolded proteins in the cell. Despite much effort over the last decades, the relative between UCH-L1 and other neurodegenerative diseases such as PO is still unclear. In other to investigate the role of UCH-L1, the authors expressed dUCH, a homolog of UCH-L1 in Drodophila, in transgenic flies and studied its function. By constructing vector pUAST-dUCH and microinjecting into Drodophila. the authors created the transgenic fly lines earring UAS-dUCH and expressed dUCH in specific tissue via GAL4-UAS system. Crossing UASdUCH lines with GMR-GAL4, the authors found that dUCH is overexpressed in posterior of eye imaginal disc, an important part to the eye development
  therefore, the overexpression of dUCH affected the eye phenotype. Moreover, transgenic flies earring UAS-dUCH ean be a model far further investigation of Parkinson disease.
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