The cross-sectional study, combined qualitative and quantitative approach was conducted among 53 secondary nurses with 2 year program in Bac Thang Long hospital in 2012. The results were shown the low adherance with safe injection procedures, none was correctedly performed all steps of procedures, and step 1 and 4 was lowest. The awareness on importance of safe infection procedures is limited, lack of knowledge on risks factors for safe infection. This was partly due to limited practicing skilles in secondary medical training. The hospitals should pay more attention to continuing education, supervision and monitoring to enhance the adhenrane to safe infection procedures.