Crude lovastatin obtained from lactonization of acid menvinolinic from the fermentation broth by Aspergillus terreus ATCC 20542 in an acidic medium should be repurified because it contained many impurities. Impure lovastatin with a purity of 93.7 percent was purified by using a solvent mixture of toluene and dichloromethane to a higher purity of 98.7 percent, then was recrystallised by isopropanol and was repurified by using a mixture of alumina and acetone to a purity of 99.5 percent. Isolation and purification using dichloromethane and toluene completely removed the impurities from the fermentation broth and lactonization process, obtained lovastatin showed a high purity and satisfied the requirements of pharmaceutical lovastatin. Additionally, the purification process was simple, effective, low-costly, using non-toxic chemicals, easily applied on pilot or industrial scale. Lovastatin analysis by using nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR, DEPT, COSY, HMBC, HSQC) and thin layer chromatography confirmed its identity with that of authentic lovastatin.