HIV/AIDS is currently a public health problem worldwide. Surveillance of HIV is needed to know epidemiology of and contribute to control of HIV/AIDS. This study aims to determine HIV infection rate as well as characteristics of HIV cases in Dak Lak province during from 2001 to 2010 based on monthly data of routine surveillance system of HIV of Dak Lak province. A total of 266,176 blood sample were tested. Of which, 1,095 sample were positive with HIV. Injecting drug users accounted for 33.7 percent, sexual workers were 0.6 percent, pregnant women were 2.4 percent, tuberculosis patients were 2.7 percent. Transmission of HIV by blood routes accounted for 41.1 percent, Sexual transmitted infection was 17.3 percent 'and mother to child was 1.6 percent. The research need to strengthen surveillance and intervention programs for harm reduction of HIV transmission in the community. In addition, measures are needed to determine more accurately as well as occupational transmission in the objects that have not been clearly defined.