A study was done on 513 patients from 15-49 years of age (266 males, 247 females) with urethral or vaginal discharge or/and genital warts in five provinces including Danang, Binhdinh, Khanhhoa, Daklak and Gialai. PCR was used as method of N.gonorrhoeae and C.trachomatis and HPV detection as well as serovars of C.trachomatis and genotypes of human papillomavirus finding. Result showed that N.gonorrhoeae, C.trachomatis and HPV infection rate were 24.36 percent and 13.84 percent and 39.57 percent, respectively. Furthermore, N.gonorrhoeae and C.trachomatis confection rate in HPV patients was 2.82 percent and 6.77 percent. The patients with urethral discharge and vaginal discharge that HPV confection rate was detected 4.56 percent and 30.93 percent. The most common serovar C.trachomatis detected in study were serovars E and F which its rate were more than serovars D, G, H, J, K. The serovars of C.trachomatis distribution in the patients of N. gonorrhoeae and HPV infection were not statistically significant differences (p0.05). The single HPV types and multiple HPV types infection rate was 8.78 percent and 30.79 percent. Only low risk HPV types infection (5.45 percent) and high risk HPV types infection (7.01 percent) and both low and high risk HPV types coinfection (27.09 percent).