Designing a microbial factory suited for plant chloroplast-derived enzymes to efficiently and green synthesize natural products: Capsanthin and capsorubin as examples.

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Tác giả: Huibin Chen, Guiping Guo, Qiaoyue Li, Zhen Liu

Ngôn ngữ: eng

Ký hiệu phân loại: 338.47001—.47999 Secondary industries and services

Thông tin xuất bản: Belgium : Metabolic engineering , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 641736

Specific cellular microenvironment, multi-enzyme complex and expensive essential cofactor make the biological manufacturing of plant chloroplast natural products (PCNPs) extremely challenging. The above difficulties have hampered the biosynthesis of capsanthin and capsorubin in the past 30 years. Here, we take capsanthin and capsorubin as examples to design an innovative microbial factory to promote the heterologous synthesis of PCPNs. Our main strategy is mimicking the microenvironment of chloroplasts in microbial factory. First, accumulation of violaxanthin, which is the key precursor, was increased by 587.9%, through introducing oxidative microenvironment and thioredoxin. The initial capsanthin-producing strain with 0.28 mg g
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