Unleashing the innate ability of Escherichia coli to produce D-Allose.

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Shota Atsumi, John Didzbalis, Bryant Luu, Dileep Sai Kumar Palur, Justin B Siegel, Jayce E Taylor

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: Belgium : Metabolic engineering , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 641921

D-allose is a rare monosaccharide, found naturally in low abundances. Due to its low-calorie profile and similar taste to sucrose, D-allose has the potential to become an ideal sugar substitute. D-allose also displays unique properties and health benefits that can be applied to various fields, including food and medicine. D-allose can be produced using two enzymatic steps in vitro: the epimerization of D-fructose, then the isomerization of the resulting D-psicose. This method suffers from poor yield due to the reversible nature of both reactions. We found that Escherichia coli possesses all of the required enzymes to convert D-glucose to D-allose with a thermodynamically favorable pathway, through a series of phosphorylation-epimerization-isomerization-dephosphorylation steps. To increase carbon flux toward D-allose production, the pathway genes were additionally expressed, and the competing pathways were removed. The engineered strains achieved production of D-allose, at a titer of 56.4 g L
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 36225755 | Email: tt.thuvien@hutech.edu.vn

Copyright @2024 THƯ VIỆN HUTECH