20 bacteria strains of gelatine analysed were screencd from fish have been symptom of slip viscosity, 8/20 strains have been identified that Pseudomonas. Two of Pseudomonas strains named C4.4.1 and C7.7.3 can synthesize gelatinase belong to group A, the activity section has a molecular weight of 18.5 kDa, the gene encoding sized 615 bp. Both of them showed the ability to grow and the activity of gelatinase get strongest level at 37-41oC in 36-48 h. Evaluation of the potential ability analyse to pig skin and gelatine powder, Pseudomonas C4.1.1 and C7.7.3 have performance to approximately 90 percent and 80 percent, respectively.