Distinct single-cell transcriptional profile in CD4+ T-lymphocytes among obese children with asthma.

• Tác giả: Ian M Adcock, Franco R D'Alessio, Nadia N Hansel, Nazanin Z Kermani, Meredith C McCormack, Karthik Suresh, Vickram Tejwani, Andres Villabona-Rueda, Rulin Wang, Tianshi David Wu, Srinivasan Yegnasubramanian, Joe Zein
• Ngôn ngữ: eng
• Ký hiệu phân loại: 627.12 Rivers and streams
• Thông tin xuất bản: United States : American journal of physiology. Lung cellular and molecular physiology , 2025
• Bộ sưu tập: NCBI
• ID: 642094

Tóm tắt

Obesity is a risk factor for asthma morbidity, associated with less responsiveness to inhaled corticosteroids. CD4+ T cells are central to the immunology of asthma and may contribute to the unique obese asthma phenotype. We sought to characterize the single-cell CD4+ transcriptional profile differences in obese children with asthma compared with normal-weight children with asthma. Eight normal-weight and obese participants with asthma were clinically phenotyped and matched based on asthma control. Peripheral blood (PB) CD4+ T cells were sorted, and single-cell RNA sequencing was conducted. Cell clusters were identified by canonical gene expression and differential gene expression and reactome pathway analysis was applied. The obese PB bulk transcriptomic signature from the U-BIOPRED pediatric cohort was assessed in our cohort as well. Obese children with asthma have a distinct CD4+ transcriptional profile with differential gene expression. There were more activated protein tyrosine phosphate receptor type C (PTPRC)