Aeromonas hydrophila is a major aquatic habitat pathogen responsible for huge economic losses in the aquaculture and food industries. In this study, a lytic bacteriophage AHPMCC11 was isolated by using A. hydrophila MTCC 1739. AHPMCC11 showed a short latent period of 10 min and the burst size was 215 PFU/cell. AHPMCC11 had potent bacteriolytic activity within 2 h in liquid culture inhibition assay and exhibited biofilm scavenging activity against A. hydrophila MTCC 1739. AHPMCC11 was found stable at a wide range of pH levels (3-12), temperature ranges (4-37 °C), and salinity conditions (0-40 ppt). The AHPMCC11 genome was determined to be 42,439 bp in length with 58.9 % G + C content, 51 CDS, and no tRNA. Comparative genome study suggested that AHPMCC11 may represent a novel species within the Autographiviridae family, belonging to the Ahphunavirus genus. In conclusion, AHPMCC11 might be used as a biocontrol agent in aquaculture and the food industry.