Nitrate, a highly reactive form of inorganic nitrogen, is commonly found in aquatic environments. Understanding the dynamics of nitrate-N concentration in rivers and its interactions with other water-quality parameters is crucial for effective freshwater ecosystem management. This study uses advanced machine learning models to analyse water quality parameters and predict nitrate-N concentrations in the lower stretch of the Ganga River from the observations of six annual periods (2017 to 2022). The parameters include water temperature, pH, specific conductivity (Sp_Con), dissolved oxygen (DO), nitrate-N, total phosphate (TP), turbidity, biochemical oxygen demand (BOD), silicate, total dissolved solids (TDS), and rainfall. The present study evaluated the predictive performance of five models-Multiple Polynomial Regression (MPR), Generalized Additive Models (GAMs), Decision Tree Regression, Random Forest (RF), and XGBoost (Extreme Gradient Boosting)-using RMSE, MAE, MAPE, NSE and R