BACKGROUND: Optimal levels and bioavailability of trace minerals (TM) in the broiler diet are important for improving performance and health status in the presence of dietary toxins. METHODS: The study examines the effectiveness of advanced chelate technology-based 7-minerals (ACTM) in broilers fed aflatoxin B1 (AFB RESULTS: The results showed that the ACTM100 and ITM + ACTM25 treatments resulted in higher average weight gain and European production efficiency index compared to the PC + ITM treatment, but lower than the NC + ITM treatment. Key indicators of gut health, such as ileal digestibility of crude fat and phosphorus, AMEn value, duodenal villus height to crypt depth ratio, villus surface area, and gene expression of junctional adhesion molecule 2 were significantly improved in the ACTM100, ITM + BT, and NC + ITM groups compared to the PC + ITM group. Additionally, jejunal occludin expression increased in the ACTM100, ITM + ACTM25, ITM + TB treatments, and the jejunal zonula occludens-1 expression increased significantly in the ACTM100 and ITM + ACTM25 groups. CONCLUSION: The results indicate that completely replacing ITM with ACTM or adding ACTM supplement to ITM diets at 25 % extra commercial levels can improve growth performance, gut health, and nutrient digestibility in the presence of AFB