Despite the prevalence of dermatophytosis which affects a quarter of the world's population, there is a pressing need for innovative and effective treatment strategies. Targeting lipid structures and their biosynthetic pathways has emerged as a promising approach to combat antimicrobial drug-resistant cases. However, our knowledge of Trichophyton-related lipids is rather limited. In this preliminary study, using mass spectrometry approach, we have determined the lipid profiles of three common Trichophyton species, namely T. rubrum, T. tonsurans, and T. interdigitale. The study presents novel findings on the comparative lipid profiles of these Trichophyton species and their observed phenotypes. These data provide a unique reference for future research on lipid homeostasis of Trichophyton-mediated dermatophytosis.