AIM: This study aimed to assess the impact of training on central venous catheter care, aligning with evidence-based guidelines, on the knowledge levels and care practices of nurses within intensive care units. METHOD: This quasi-experimental study was conducted with the voluntary participation of 39 nurses from a state hospital's intensive care units. The data were collected using the "Descriptive Characteristics Form," the "Central Venous Catheter Information Form for Evidence-Based Guidelines," the "Observation Form for the Evaluation of Nurse Care Practices in Central Venous Catheter Care Guided by Evidence-Based Guidelines," and the "Evidence-Based Nursing Attitude Questionnaire". Nurses underwent Central Venous Catheter Care Training, and assessments of their knowledge levels and care practices were conducted before and after the training. RESULTS: Following the central venous catheter care training, there was a significant increase in posttest scores for both the knowledge level (p <
0.001) and the Evidence-Based Nursing Attitude Questionnaire (p <
0.001). CONCLUSION: This study suggests the incorporation of central venous catheter care based on evidence-based guidelines into nursing curricula, postgraduate in-service training programs, and intensive care courses.