Evolutionarily, Wnt/β-catenin signaling is well-conserved and supports several key cell-biological processes (e.g. adhesion and proliferation). Its crucial component, β-catenin, has been described in several organisms, however, its identification and characterization are notably lacking in annelid earthworms. Here, we report a novel β-catenin homologue from the earthworm Eisenia andrei, termed Ea-β-catenin. The full-length 3253 nt Ea-β-catenin mRNA includes an open reading frame of 2499 nt encoding a putative protein with 833 amino acid residues that comprise 11 classical armadillo-repeat regions. Phylogenetic analysis indicates that Ea-β-catenin shows strong homology with Lophotrochozoan β-catenins. Ubiquitous, but variable expressions of Ea-β-catenin were observed in distinct earthworm tissues. During embryogenesis, Ea-β-catenin mRNA gradually increased from the E1 to E4 developmental stages. Regeneration experiments revealed an inverse correlation between Ea-β-catenin mRNA levels and the rate of EdU