Extraction, optimization, purification and characterization of inulin from chicory roots using conventional and greener extraction techniques.

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Tác giả: Harish Kumar Chopra, Himanshu Rani, Mahendra Singh

Ngôn ngữ: eng

Ký hiệu phân loại: 739.2 Work in precious metals

Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 643747

Inulin is a natural polysaccharide that has abundant applications in the chemical, food, and pharmaceutical industries. In the present studies, extraction of inulin was attained using conventional techniques (Soxhlet) and green techniques (ultrasonication) from chicory roots. The extraction parameters were optimized using Box-Behnken design and response surface methodology (RSM). The parameters selected were ultrasonication temperature (40-80 °C), time duration (30-120 min), and solid-to-solvent ratio (1:10-1:40 g/mL). Distilled water was used as solvent. The optimal conditions for extracting inulin using the UAE method were solid-to-solvent ratio of 1:40 g/mL, 120 min, extraction temperature of 60 °C, to get maximum yield (64.79 %). For the Soxhlet apparatus method, these conditions were 1:40 g/mL, 6 h, 90 °C, and (59.1 %), respectively. LC-MS showed maximum purity (98 %) of inulin in the UAE method. FT-IR,
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