Production and Characterization of Copolymers Consisting of 3-Hydroxybutyrate and Increased 3-Hydroxyvalerate by β-Oxidation Weakened Halomonas.

• Tác giả: Guo-Qiang Chen, Jiangnan Chen, Hongtao He, Yunyun Ouyang, Helen Park, Huan Wang, Fuqing Wu, Fang Yang, Weinan Yang, Yiping Yuan
• Ngôn ngữ: eng
• Thông tin xuất bản: Belgium : Metabolic engineering , 2025
• Bộ sưu tập: NCBI
• ID: 643826

Tóm tắt

Polyhydroxyalkanoates (PHA) with high 3-hydroxyvalerate (3HV) monomer ratios lead to their accelerated biodegradation and improved thermal and mechanical properties. In this study, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) with a broad range of 3HV ratios were produced and characterized using the next generation industrial biotechnology (NGIB) chassis Halomonas bluephagenesis (H. bluephagenesis). Wild type H. bluephagenesis was found to produce P(3HB-co-66.31mol% 3HV) when cultured in the presence of valerate. Deletion on the functional enoyl-CoA hydratase (fadB