Characterisation of MRGPRX2

• Tác giả: Guy Boeckxstaens, Stuart Brierley, María Cuende Estévez, Elisabetta De Marco, Lisse Decraecker, Alexandre Denadai-Souza, Andrea Fanjul, Sonia Garcia Caraballo, Tony Gibson, Gareth Hicks, Hind Hussein, Runze Quan, Nathalie Stakenborg, Yasuhiro Tsukimi, Samuel Van Remoortel, Pieter Vanden Berghe, Maria Francesca Viola, Zheng Wang, Wendy Winchester, Mira Wouters, Jill Wykosky
• Ngôn ngữ: eng
• Ký hiệu phân loại: 597.959 *Anguinomorphoidea
• Thông tin xuất bản: England : Gut , 2025
• Bộ sưu tập: NCBI
• ID: 644103

Tóm tắt

BACKGROUND: Mast cell activation is an important driver of abdominal pain in irritable bowel syndrome (IBS). While evidence supports the role of IgE-mediated mast cell activation in visceral pain development in IBS, the role of pseudoallergic MRGPRX2-mediated mast cell activation in this process remains unknown. OBJECTIVE: We investigated whether MRGPRX2-mediated mast cell activation plays a role in abdominal pain development in patients with IBS. DESIGN: MRGPRX2 expression in mast cells and other immune cells was characterised across colon layers using flow cytometry. We evaluated whether MRGPRX2 agonists trigger mast cell degranulation and transient receptor potential vanilloid 1 (TRPV1) sensitisation in healthy human colonic submucosal plexus samples using live imaging. Rectal biopsies were then collected from patients with IBS and healthy volunteers (HV) and MRGPRX2 RESULTS: MRGPRX2 CONCLUSION: The MRGPRX2 pathway is functionally upregulated in the colon of patients with IBS, supporting its role in abdominal pain in IBS.