Ứng dụng công nghệ adn để phân loại và nhận diện lan hoàng thảo trầm rừng (dendrobium parishii) và phi điệp (dendrobium anosmum) tại việt nam

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Tác giả: Thành Trí Đỗ, Duy Dương Trần, Hoàng Dũng Trần, Lệ Trúc Hà Trần, Thị Huyền Trang Vũ

Ngôn ngữ: vie

Ký hiệu phân loại: 332.6457 Investment

Thông tin xuất bản: Nông nghiệp & Phát triển nông thôn, 2012

Mô tả vật lý: 45360

Bộ sưu tập: Metadata

ID: 645102

Thêm vào giỏ

Several of the orchid species with a powerful fragrance such as Dendrobium parishii and Dendrobium anosmum have attracted the attention of botanists because of their unique biological characters. The identification of species based on morphology is difficult even though they are flowering. In this study, 10 samples of Dendrobium parishii and Dendrobium anosmum were collected and differenced by the sequences of partial rDNA (ribosomal DNA). Nine of them were successfully to amplify and sequences for ITS1, 5.8S and ITS2 (internal transcribed spacer) region in which the lengths of PCR products are 750 to 850 bp. The phylogenetic analysis showed that the ITS sequences help to resolve the position of D. parishii and D. anosmum undoubtedly in the trees. Several samples were revised from Dendrobium parishii to Dendrobium anosmum according to their ITS sequences identify and position in the evolutionary trees.

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