Using chemicals pyrethroid group to treat bed nets and residual spraying in our country are expanding the superiority Vietnam, effectively in preventing individuals. However, a number of mosquito species transmit malaria appear to endure expression and resistance to pyrethroid chemical groups. Early detection and accurate level of insecticide resistance of malaria transmission is essential. The application of molecular biology techniques in the study of insecticide resistance is growing stronger around the world to find out sooner effective measures for the prevention of mosquito species. Application realtime-PCR technique in detecting chemical resistant mosquitoes showed the advantages of this method as early detection, rappid and accurate.