As of 2010, it is estimated that there are 36 million people living with HIV worldwide and more than 15,000 new infections are occurring daily. Immunodeficiency virus (HIV) has long been a major problem causing mortality and morbidity in Vietnam. According to the Joint United Nations Programme on HIV/AIDS, the HIV epidemic in Viet Nam is still in a concentrated stage, with the highest HIV prevalence found in injecting drugs users (IDU), female sex workers (FSW) and men who have sex with men (MSM). Our previous study has shown that subtype CRF01_AE is predominant in Vietnam similar to the case in Thailand, whereas it was found to be minority in other countries. Early detection of HIV has important implications for both prevention and treatment. The aim of this study was to develop a Latex kit to be performed in laboratories with serum specimens without the need for sophisticated equipments as well as skillful technicians. In order to achieve this goal, genes coding for the envelope (gp120, gp41) and the core (p24) proteins of subtype CRF01_AE/HIV-1 were cloned, expressed in E. coli and purified. The purified recombinant proteins were then used to develop Latex kit for detection of antibodies to HIV-1 in HIV patients. The sensitivity and specificity of the Latex kit was further characterized with 297 clinical samples.. The result showed a sensitivity and specificity of 97.6 and 98,6 percent respectively.