Staphylococcus aureus is the cause of many different infections. In addition, enterotoxigenic S. aureus is one of the major pathogens causing food poisoning cases world-wide. Detection of enterotoxin genes plays an important role in diagnosis, study on pathogenesis and molecular epidemiology. The study was carried out to detect enterotoxin gene distribution in Staphylococcus aureus strains isolated from noses and hands of staffs working in restaurants in Hqnoi. The result show that the genes coding for enterotoxin C and E, sec, and see were the most frequent (23.1 percent), followed by seg (21.2 percent), sei (15.4 percent), sea (11.5 percent), sed (3.8 percent) and seb (1.9 percent). Non of strains had sej 30 percent of the strains had only one enterotoxin gene, 40 percent carried two genes coding for enterotoxins, 16.7 percent were positive for three genes. Genotypes encoding four enterotoxins were detected in 3.3 percent of the strains. Conclusion: 90 percent of strains carried genes coding for enterotoxins. Among the genes that code for classic enterotaxins (SEA-SEE), sec and see were the most frequent. They were found in 23.1 percent of the strains. The highest prevalence of the new enterotoxins was seg (21.2 percent), followed by sei (15.4 percent). 60 percent of the strains carried more than one gene encoding the enterotoxins.