OBJECTIVE: This trial probed the correlation between miR-31 expression and endometrial receptivity (ER) in patients with repeated implantation failure (RIF) of in vitro fertilization and embryo transfer (IVF-ET). METHODS: A retrospective study of 80 infertility patients who underwent IVF-ET assisted conception treatment were divided into RIF group and normal pregnancy group (control group) according to the pregnancy outcome after embryo transfer. General information of both groups was collected. Endometrial tissues were collected in the middle luteal phase of the menstrual cycle before IVF-ET. miR-31 levels in endometrial tissues were measured, and endometrial tolerance indicator pulsatility index (PI), resistance index (RI), and endometrial thickness (Em) were detected. The correlation between endometrial miR-31 levels and ER indices was evaluated by Pearson method. ROC curves were utilized to analyze the efficacy of miR-31 in predicting RIF occurrence. The influencing factors of RIF were analyzed by binary Logistic regression. RESULTS: RIF patients had increased miR-31 expression level and endometrial tolerance indicator PI, and RI while decreased Em ( CONCLUSION: miR-31 in RIF patients is positively correlated with PI and RI, and negatively correlated with Em.