Shift workers have a 40% higher risk for cardiovascular disease (CVD) compared to people who work day shifts. However, the acute impact of shift work on CVD risk factors in free-living settings remains unclear. We therefore investigated the impact of acute night shift work on factors related to cardiovascular health including blood pressure (BP) and sleep duration. Twenty-four rotating shift workers (19F, 23 ± 4 y, BMI: 23 ± 3 kg/m