Study on the regeneration capacity of notoginseng (Stablianthus thorelli Gagnep) was performed on MS (Murashige Skoog) medium that supplemented various growth regulations (BAP, kinetin, TDZ, and NAA). The results showed that the highest of sterilization efficiency of tubers (71.11 percent) was obtained at treatment of HgCl2 0.1 percent for 10min. The efficiency of shoot regeneration was the highest on MS medium with BAP 5.0mg/l of supplement, 108 shoots were generated after 14 days of culture. The effect of BAP, kinetin, TDZ, and NAA on shoot multiplication of notoginseng were determined: (i) supplement of BAP 5.0mg/l and NAA 0.5mg/l into MS medium could be generated 4.31 shoots/sample
(ii) the number of shoot regeneration was increased when adding of either kinetin 0.3mg/l or TDZ 0.5mg/l into MS medium containing BAP 5.0mg/l and NAA 0.5mg/l, 4.71 and 5,11 shoots/sample respectively. Plants were then transplanted in mixture soil (soil and rice husk, 3:1).