The authors have been researching and manufacturing rapid diagnostic ELISA KIT Gumboro disease, results showed that: concentrations of antigen Gumboro is 25 micrograms in one ml, dilution of serous I: 200 is the appropriate level for ELISA reaction to detecting specific antibodies of Gumboro virus. Appropriate time incubate with antibodies specific antigen of Gumboro virus and anti-bovine IgG: HRPO is 90 minutes, incubated anti-bovine IgG: HRPO complex with the antibody - antigen is 45 minutes. Antibody dilution and antibody incubation time appropriate equivalent is I: 200 and 90 minutes. Dilution of antibodies and antibody incubation period is appropriated equivalent of 1: 200 and 90 minutes. ELISA plate washing time is 3 times, one minute for each time. This result is the basis for conducting the next step in the creation of rapid diagnostic KIT Gumboro disease.