Although it is not a new field nowadays but fermentation technology of antibiotics has developed robustly and played an important role in modem medicine which has been using antibiotics for diseases treatment. In the study, the authors described the results which related to the optimal conditions for the antibiotic extraction from strain of actinomycetes Streptomyces olivaceus TT1.2. As the result, this strain showed the best fermentation possibility in A4-H medium and 168 hours were suitable for antibiotic biosynthesis at pH = 7. The antibiotic extracted from TT1.2 strain could be soluble well in different solvents and much better in some solvents such as Chloroform, ethyl acetate, toluene, 3 - methyl butanol. The pH values for extraction of antibiotics ranged from 3 to and the most suitable pH was about pH = 3. According to practical conditions, experimental purposes and standards of safety, the authors selected 3 - methyl butanol for further studies. The results showed that the ratio of solvent and fermented solution as 1:3 respectively was the best choice for antibiotic extraction. The antibiotic extracted from TT1.2 strain seem to be unaffected much by high heat, especially heated at 100oC for 60 minutes the activity of antibiotic remained 94.4 percent (for Salmonella typhi) and 72.7 percent (for Shigella jlexneri ) compared to negative controls. Moreover, this antibiotic was stable in the range of pH variations from 4 to 9, antimicrobial activity increased maximally at pH = 7 in all cases of using 3 examined microorganism strains.